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The gene expression profile of the small intestine from mice following ABI. Mice were treated with melatonin (10 mg/kg) daily for 5 consecutive days by intraperitoneal injection before exposure to a dose of 14 Gy ABI. The small intestines were harvested at 3 days after radiation and DNA <t>microarray</t> analysis was performed. ( A ) The number of upregulated DEGs and downregulated DEGs for mice. The red-and-green chart shows the upregulated DEGs (red) and the downregulated DEGs (green). ( B ) Cluster analysis of similarity of genome-wide expression for mice. Every row represents a piece of chip; every line represents a gene probe. The red and green levels of probe signals indicate the level of upregulation (red) or downregulation (green). ( C ) Significantly enriched GO analysis (top10) of the DEGs for mice. The values of −log10 ( p -value) and −log10 (Q-value) show the relationship between gene expression and the relevant GO category. The y -axis represents the GO category and the x axis the −log10 ( p -value) and −log10 (Q-value). n = 3 mice/group.
Mouse Oligo Microarray Mouse Whole Genome Onearray Moa2.1, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The gene expression profile of the small intestine from mice following ABI. Mice were treated with melatonin (10 mg/kg) daily for 5 consecutive days by intraperitoneal injection before exposure to a dose of 14 Gy ABI. The small intestines were harvested at 3 days after radiation and DNA microarray analysis was performed. ( A ) The number of upregulated DEGs and downregulated DEGs for mice. The red-and-green chart shows the upregulated DEGs (red) and the downregulated DEGs (green). ( B ) Cluster analysis of similarity of genome-wide expression for mice. Every row represents a piece of chip; every line represents a gene probe. The red and green levels of probe signals indicate the level of upregulation (red) or downregulation (green). ( C ) Significantly enriched GO analysis (top10) of the DEGs for mice. The values of −log10 ( p -value) and −log10 (Q-value) show the relationship between gene expression and the relevant GO category. The y -axis represents the GO category and the x axis the −log10 ( p -value) and −log10 (Q-value). n = 3 mice/group.

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Melatonin Administration on Intestinal Injury Caused by Abdominal Irradiation from Mice

doi: 10.3390/ijms22189715

Figure Lengend Snippet: The gene expression profile of the small intestine from mice following ABI. Mice were treated with melatonin (10 mg/kg) daily for 5 consecutive days by intraperitoneal injection before exposure to a dose of 14 Gy ABI. The small intestines were harvested at 3 days after radiation and DNA microarray analysis was performed. ( A ) The number of upregulated DEGs and downregulated DEGs for mice. The red-and-green chart shows the upregulated DEGs (red) and the downregulated DEGs (green). ( B ) Cluster analysis of similarity of genome-wide expression for mice. Every row represents a piece of chip; every line represents a gene probe. The red and green levels of probe signals indicate the level of upregulation (red) or downregulation (green). ( C ) Significantly enriched GO analysis (top10) of the DEGs for mice. The values of −log10 ( p -value) and −log10 (Q-value) show the relationship between gene expression and the relevant GO category. The y -axis represents the GO category and the x axis the −log10 ( p -value) and −log10 (Q-value). n = 3 mice/group.

Article Snippet: The aminoallyl-RNA probes were labelled with Cy5 hybridized for 16 h at 50 °C to a mouse oligo microarray (Mouse Whole Genome OneArray MOA2.1; Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Gene Expression, Injection, Microarray, Genome Wide, Expressing

qRT-PCR validation of seven candidate DEGs. ( A ) The expression levels of the target genes of TRIM24 and S100A9 in the microarray data. ( B ) The expression levels of the target genes of TRIM24 and S100A9 validated in the qRT-PCR. Data are represented as mean ± SD, n = 3 mice/group. a p < 0.05 vs. Ctr; b p < 0.05 vs. Rad. NOS2, nitric oxide synthase 2, inducible; VEGFA, vascular endothelial growth factor A; DDC, dopa decarboxylase; VCAN, versican; LGALS3, lectin, galactose binding, soluble 3; IFNG, interferon gamma; TLR2, toll-like receptor 2.

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Melatonin Administration on Intestinal Injury Caused by Abdominal Irradiation from Mice

doi: 10.3390/ijms22189715

Figure Lengend Snippet: qRT-PCR validation of seven candidate DEGs. ( A ) The expression levels of the target genes of TRIM24 and S100A9 in the microarray data. ( B ) The expression levels of the target genes of TRIM24 and S100A9 validated in the qRT-PCR. Data are represented as mean ± SD, n = 3 mice/group. a p < 0.05 vs. Ctr; b p < 0.05 vs. Rad. NOS2, nitric oxide synthase 2, inducible; VEGFA, vascular endothelial growth factor A; DDC, dopa decarboxylase; VCAN, versican; LGALS3, lectin, galactose binding, soluble 3; IFNG, interferon gamma; TLR2, toll-like receptor 2.

Article Snippet: The aminoallyl-RNA probes were labelled with Cy5 hybridized for 16 h at 50 °C to a mouse oligo microarray (Mouse Whole Genome OneArray MOA2.1; Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray, Binding Assay